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Related Experiment Video

Updated: Jun 22, 2026

Assessment of Viability of Human Fat Injection into Nude Mice with Micro-Computed Tomography
11:13

Assessment of Viability of Human Fat Injection into Nude Mice with Micro-Computed Tomography

Published on: January 7, 2015

A murine model for studying diffusely injected human fat.

Vishal D Thanik1, Christopher C Chang, Oren Z Lerman

  • 1New York, N.Y. From the New York University School of Medicine.

Plastic and Reconstructive Surgery
|July 2, 2009
PubMed
Summary
This summary is machine-generated.

A new mouse model successfully replicates human fat grafting, showing high fat persistence and vascularization with minimal inflammation. This model aids in studying fat graft survival and progenitor cells.

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Related Experiment Videos

Last Updated: Jun 22, 2026

Assessment of Viability of Human Fat Injection into Nude Mice with Micro-Computed Tomography
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A Microphysiologic Platform for Human Fat: Sandwiched White Adipose Tissue
08:14

A Microphysiologic Platform for Human Fat: Sandwiched White Adipose Tissue

Published on: August 15, 2018

Area of Science:

  • Regenerative Medicine
  • Tissue Engineering
  • Adipose Tissue Biology

Background:

  • Human autologous fat grafting studies have been largely anecdotal.
  • A need exists for a reproducible model to study fat graft fate and cellular components.
  • This study introduces a novel murine model for investigating human fat grafting.

Purpose of the Study:

  • To develop and validate a murine model that accurately recapitulates human fat grafting.
  • To investigate the survival, vascularization, and cellular behavior of grafted human adipose tissue in a host environment.
  • To establish a platform for future research on adipose-derived progenitor cells.

Main Methods:

  • Human lipoaspirate was injected into nude and tie2/lacZ mice using a standardized technique.
  • Tissue samples were collected at various time points and analyzed using histological staining (H&E, Cytochrome c oxidase IV, X-galactosidase).
  • Volumetric analysis was performed at 8 weeks to assess fat graft persistence.

Main Results:

  • The murine model demonstrated 82% persistence of grafted human fat volume at 8 weeks.
  • Histological analysis revealed viable adipocytes with minimal inflammatory or capsular response.
  • Vascularization of the fat graft was confirmed, with human-derived blood vessels identified by specific staining.

Conclusions:

  • A validated murine model for human fat grafting has been established.
  • The model shows high fat graft viability, persistence, and integration with host vasculature.
  • This platform enables further investigation into the role of progenitor cells in lipoaspirate for regenerative purposes.