Related Experiment Video
Updated: Jun 22, 2026

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
Published on: May 30, 2016
Stochastic transfer function for structured illumination microscopy
Michael G Somekh1, Ken Hsu, Mark C Pitter
1Institute of Biophysics Imaging and Optical Science, The University of Nottingham, University Park, Nottingham, UK. mike.somekh@nottingham.ac.uk
Abstract:
The stochastic transfer function extends the concept of the conventional transfer function by incorporating noise statistics, thus giving a measure of the signal-to-noise ratio at each spatial frequency. This provides a convenient and standardized metric to assess the trade-off in terms of spatial frequency bandwidth and signal-to-noise ratio for a diverse range of resolution enhancement techniques. We apply this concept to structured illumination microscopy and compare its noise performance as a function of frequency with the conventional wide-field fluorescent microscope. The result suggests how a hybrid algorithm further improves the photon efficiency of the structured illumination technique.
More Related Videos
14:09High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip
Published on: November 16, 2019
08:17Probing Structural and Dynamic Properties of Trafficking Subcellular Nanostructures by Spatiotemporal Fluctuation Spectroscopy
Published on: August 16, 2021
Related Concept Videos
Super-resolution Fluorescence Microscopy
Phase Contrast and Differential Interference Contrast Microscopy
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
Confocal Fluorescence Microscopy