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Cell-based in vivo dual imaging probes using genetically expressed tags and chemical contrast agents

Yuki Hasegawa1, Aki Honda, Keitaro Umezawa

  • 1Department of Applied Chemistry, Faculty of Science and Technology, Keio University, Kohoku-ku, Yokohama, 223-8522, Japan.

Chemical Communications (Cambridge, England)
|July 2, 2009
PubMed

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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...