Pulsatile insulin delivery through the ocular route

A liposomal system bearing insulin was developed for controlled and prolonged delivery of drug through the ocular route. The systems were prepared by the cast film method using various lipids in different weight ratios. These products were characterized by their physical attributes, viz., shape, size, charge, and encapsulation efficiency. The effect of temperature on the formulation was also studied by measuring the shape and drug content. An in vitro drug release study was carried out using a franz diffusion cell and artificial tear solution as diffusion medium. An in vivo experiment was conducted on rabbits by periodic monitoring the blood glucose level after the instillation of the product in the animal's eye. The results indicate the potentiality of the liposomal system for controlled delivery of insulin for prolonged periods of time. Thus, pulsatile delivery of insulin can be achieved using temperature-sensitive liposomal formulation.