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Paper microzone plates.

Emanuel Carrilho1, Scott T Phillips, Sarah J Vella

  • 1Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, Massachusetts 02138, USA. emanuel@iqsc.usp.br

Analytical Chemistry
|July 4, 2009
PubMed
Summary
This summary is machine-generated.

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Researchers developed inexpensive, paper-based microzone plates as a novel alternative to plastic well plates. These paper plates enable sensitive fluorescence and absorbance measurements with reduced sample volumes, offering new capabilities for bioassays.

Area of Science:

  • Biomaterials engineering
  • Analytical chemistry
  • Microfluidics

Background:

  • Conventional multiwell plates are typically fabricated from polymers.
  • There is a need for low-cost, high-performance alternatives for bioassays.

Purpose of the Study:

  • To develop and characterize novel paper-based microzone plates.
  • To evaluate their performance in quantitative absorbance and fluorescence measurements.
  • To demonstrate their potential as a cost-effective alternative to plastic plates.

Main Methods:

  • Fabrication of paper-based microzone plates using photolithography with a rapid photoresist formulation.
  • Patterning of paper into hydrophilic zones surrounded by hydrophobic barriers.
  • Quantitative absorbance and fluorescence measurements using conventional microplate readers.

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  • Image analysis using scanners or cameras for colorimetric assays.

    • Main Results:

    • Paper-based plates are thin (180 microm) and require small sample volumes (5 microL).
    • Low limits of detection achieved for fluorescence (125 fmol) and absorbance (50 pmol).
    • Demonstrated compatibility with standard microplate readers and potential for reader-free assays.

    Conclusions:

    • Paper-based microzone plates offer a low-cost ($0.05/plate) and efficient platform for bioassays.
    • These plates provide new capabilities and are suitable for quantitative measurements.
    • They represent a promising alternative to conventional polymer-based multiwell plates.