Metal ion CHElate-aSSisted LIGAtion (CHESS LIGA) for SNP detection on microarrays
Thi Hien Le1, Tatiana S Oretskaya, Timofei S Zatsepin
1Chemistry Department, M.V. Lomonossov Moscow State University, Leninskije Gory, Moscow 119991, Russia.
Bioorganic & Medicinal Chemistry Letters
|July 4, 2009
Abstract:
We developed a metal ion chelate-assisted ligation for SNP detection by microarray. An oligonucleotide probe was separated into two 9-10-mers bearing iminodiacetic residues at the gap point. Duplex formation with the DNA target was possible only if nickel ions were added, but a nucleotide substitution opposite the gap point prevented duplex formation. Here we demonstrate the application of this approach for SNP detection (A1298C) within the 5,10-methylenetetrahydrofolate reductase gene on a microarray.
