Related Experiment Video
Updated: Jun 21, 2026

08:47
Microdialysis of Excitatory Amino Acids During EEG Recordings in Freely Moving Rats
Published on: November 8, 2018
Detection and quantification of neurotransmitters in dialysates
Agustin Zapata1, Vladimir I Chefer, Toni S Shippenberg
1NIH, NIDA Intramural Research Program, Baltimore, Maryland, USA.
Current Protocols in Neuroscience
|July 4, 2009
Abstract:
Sensitive analytical methods are needed for the separation and quantification of neurotransmitters obtained in microdialysate studies. This unit describes methods that permit quantification of nanomolar concentrations of monoamines and their metabolites (high-pressure liquid chromatography electrochemical detection), acetylcholine (HPLC-coupled to an enzyme reactor), and amino acids (HPLC-fluorescence detection; capillary electrophoresis with laser-induced fluorescence detection).

