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Related Concept Videos

Spectrophotometry: Introduction01:16

Spectrophotometry: Introduction

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Spectrophotometry is the quantitative measurement of the absorption, reflection, diffraction, or transmission of electromagnetic radiation through a material as a function of the intensity and wavelength of the radiation. A spectrophotometer is a device used to measure the change in the radiation intensity caused by its interaction with the material.
The essential components of a spectrophotometer include a source of electromagnetic radiation, a slot for placing a material to be analyzed, and a...
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UV–Vis Spectrometers01:14

UV–Vis Spectrometers

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The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV radiation, and tungsten lamps, which produce radiation in the visible region, are used as light sources in UV–visible spectrophotometers. A monochromator or prism is used for diffraction grating, i.e., to split the incoming radiation into different wavelengths. A system of slits is used to focus the desired wavelength on the sample cell.
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UV–Vis Spectroscopy: Beer–Lambert Law01:09

UV–Vis Spectroscopy: Beer–Lambert Law

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The Beer-Lambert law describes the relationship between absorbance and concentration, which combines the principles established by scientists Johann Heinrich Lambert and August Beer. Lambert's law states that when light passes through a medium, the loss in intensity is directly proportional to the original intensity and the path length of the light. Beer's law proposed that the transmittance of a solution remains constant if the product of concentration and path length is constant. The modern...
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Ultraviolet and Visible (UV–Vis) Spectroscopy: Overview01:02

Ultraviolet and Visible (UV–Vis) Spectroscopy: Overview

3.9K
Ultraviolet–visible (UV–visible or UV–Vis) spectroscopy is an analytical technique that investigates the interaction between matter and UV–Vis light within the electromagnetic spectrum. This method is widely used for its versatility, simplicity, and relatively quick data acquisition, making it valuable for both qualitative and quantitative analysis. When UV–Vis radiation passes through a material,  molecules absorb light depending on the energy required for...
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UV–Vis Spectroscopy of Conjugated Systems01:32

UV–Vis Spectroscopy of Conjugated Systems

7.8K
Organic compounds with conjugated double bonds show strong absorption features in the UV–visible region of the electromagnetic spectrum attributed to π → π* electronic excitations. Generally, a UV–vis absorption spectrum is recorded as a plot of absorbance vs wavelength. The wavelength of maximum absorbance, which manifests as a peak in the absorption spectrum, is denoted as λmax.
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UV–Vis Spectroscopy: Woodward–Fieser Rules01:29

UV–Vis Spectroscopy: Woodward–Fieser Rules

27.1K
UV–Visible absorption spectra of conjugated dienes arise from the lowest energy π → π* transitions. The light-absorbing part of the molecule is called the chromophore, and the substituents directly attached to the chromophore are called auxochromes. A strong correlation exists between the absorption maxima, λmax, and the structure of a conjugated π system. The Woodward–Fieser rules predict the value of λmax for a given structure by adding the...
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Related Experiment Videos

[Study on the multiwavelength coefficient spectrophotometry and its application].

S H Huang1

  • 1Shanghai Jinshan Conunty Institute for Drug Control.

Yao Xue Xue Bao = Acta Pharmaceutica Sinica
|January 1, 1991
PubMed
Summary
This summary is machine-generated.

This study introduces a multi-wavelength coefficient method for analyzing mixed components. The method accurately quantifies mixtures like chlorpromazine hydrochloride and promazine hydrochloride, showing good precision and accuracy.

Related Experiment Videos

Area of Science:

  • Analytical Chemistry
  • Spectrophotometry

Context:

  • Simultaneous determination of multiple components in a mixture presents analytical challenges.
  • Existing spectrophotometric methods may lack specificity for complex systems.

Purpose:

  • To present a general principle of multi-wavelength spectrophotometry.
  • To introduce and validate a novel multi-wavelength coefficient method for analyzing mixed component systems.
  • To determine the coefficients and their relationship with the analytical method.

Summary:

  • A multi-wavelength coefficient method was developed based on a general principle of multi-wavelength spectrophotometry.
  • The method was applied to simultaneously assay chlorpromazine hydrochloride and promazine hydrochloride mixtures.
  • Determination of coefficients and their impact on the assay were investigated.

Impact:

  • The developed method demonstrates good precision and accuracy for simultaneous analysis.
  • The findings suggest that different coefficient sets do not significantly affect the method's performance.
  • This approach offers a robust tool for analyzing complex mixtures in pharmaceutical and chemical analysis.