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Fixation and Sectioning

Fixation and Sectioning

Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...

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Related Experiment Video

Updated: Jun 21, 2026

Undecalcified Bone Preparation for Histology, Histomorphometry and Fluorochrome Analysis

Undecalcified Bone Preparation for Histology, Histomorphometry and Fluorochrome Analysis

Published on: January 8, 2010

A method for marking histopathological specimens of neck--technical note.

N Papadogeorgakis¹, E Parara, H Pappa

¹Oral and Maxillofacial Department, Evangelismos General Hospital, University of Athens, Athens, Greece.

International Journal of Oral and Maxillofacial Surgery

|July 7, 2009

Summary

Accurate marking of neck lymph nodes aids precise histopathological examination. This improves cancer management for head and neck neoplastic diseases.

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Area of Science:

Surgical pathology
Head and neck oncology

Background:

Accurate lymph node staging is critical for head and neck cancer management.
Histopathological examination relies on precise specimen identification.

Purpose of the Study:

To describe a technical method for accurately marking neck lymph node locations.
To enhance the precision of histopathological reporting for head and neck neoplasms.

Main Methods:

A technical note detailing a specific marking technique for lymph nodes.
Focus on precise anatomical localization for surgical specimens.

Main Results:

The described method facilitates accurate identification of lymph node origins.
Improved specimen orientation for histopathological analysis.

Conclusions:

Precise lymph node marking leads to more accurate histopathological reports.
Enhanced reporting supports more effective patient management in head and neck oncology.