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Updated: Jun 21, 2026

In Vivo Targeted Expression of Optogenetic Proteins Using Silk/AAV Films
06:11

In Vivo Targeted Expression of Optogenetic Proteins Using Silk/AAV Films

Published on: February 26, 2019

Bioengineered silk protein-based gene delivery systems.

Keiji Numata1, Balajikarthick Subramanian, Heather A Currie

  • 1Department of Biomedical Engineering, Tufts University, 4 Colby Street, Medford, MA 02155, USA.

Biomaterials
|July 7, 2009
PubMed
Summary
This summary is machine-generated.

Bioengineered silk proteins form complexes with DNA for effective gene delivery. These novel silk-polylysine materials show high transfection efficiency in human embryonic kidney cells.

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Manufacture and Drug Delivery Applications of Silk Nanoparticles
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Manufacture and Drug Delivery Applications of Silk Nanoparticles

Published on: October 8, 2016

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Last Updated: Jun 21, 2026

In Vivo Targeted Expression of Optogenetic Proteins Using Silk/AAV Films
06:11

In Vivo Targeted Expression of Optogenetic Proteins Using Silk/AAV Films

Published on: February 26, 2019

Manufacture and Drug Delivery Applications of Silk Nanoparticles
09:03

Manufacture and Drug Delivery Applications of Silk Nanoparticles

Published on: October 8, 2016

Area of Science:

  • Biomaterials Science
  • Molecular Biology
  • Genetic Engineering

Background:

  • Silk proteins offer a biodegradable and non-cytotoxic platform for biomedical applications.
  • Genetic engineering allows for tailoring silk protein properties, including chemistry and molecular weight.
  • Silk's self-assembly into robust structures suggests potential for controlled delivery systems.

Purpose of the Study:

  • To bioengineer silk-based block copolymers with poly(L-lysine) domains for gene delivery.
  • To investigate the complexation of these silk-polylysine copolymers with plasmid DNA (pDNA).
  • To evaluate the gene delivery efficiency of these novel materials in human embryonic kidney (HEK) cells.

Main Methods:

  • Silk-polylysine block copolymers were synthesized via genetic engineering.
  • Ionic complexes of copolymers and pDNA were formed and characterized using agarose gel electrophoresis, atomic force microscopy, and dynamic light scattering.
  • Transfection efficiency was assessed in HEK cells.

Main Results:

  • Silk-polylysine copolymers self-assembled and effectively complexed pDNA through ionic interactions.
  • pDNA complexes with 30 lysine residues at a polymer/nucleotide ratio of 10, with a 380 nm diameter, exhibited optimal transfection efficiency.
  • Immobilization of pDNA complexes on silk films enabled direct cell transfection from surfaces.

Conclusions:

  • Bioengineered silk proteins represent a promising new class of tailored gene delivery vehicles.
  • Silk-polylysine block copolymers offer a versatile platform for efficient and controllable gene delivery.
  • The ability to immobilize complexes on silk films opens avenues for surface-mediated gene transfection.