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Updated: Jun 21, 2026

The Microfluidic Probe: Operation and Use for Localized Surface Processing
08:07

The Microfluidic Probe: Operation and Use for Localized Surface Processing

Published on: June 4, 2009

The microfluidic probe: operation and use for localized surface processing.

Cecile M Perrault1, Mohammad A Qasaimeh, David Juncker

  • 1Department of Biomedical Engineering, McGill University.

Journal of Visualized Experiments : Jove
|July 7, 2009
PubMed
Summary
This summary is machine-generated.

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The microfluidic probe (MFP) offers a novel approach for precise reagent delivery and localized processing of samples. This innovative tool overcomes limitations of traditional microfluidic devices and micropipetting systems for enhanced experimental control.

Area of Science:

  • Biotechnology
  • Microfluidics
  • Cell Biology

Background:

  • Traditional microfluidic devices utilize closed channels, limiting sample volume and accessibility.
  • Micropipetting systems offer local perfusion but lack precise 3D flow confinement and are fragile.
  • Existing methods struggle with dynamic, large-area sample processing.

Purpose of the Study:

  • To introduce and demonstrate the capabilities of the microfluidic probe (MFP).
  • To present a method for precise, localized delivery and scanning of reagents onto surfaces.
  • To overcome the limitations of conventional microfluidic and micropipetting techniques.

Main Methods:

  • The microfluidic probe (MFP) utilizes closely spaced injection and aspiration openings to create a confined microjet.

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Last Updated: Jun 21, 2026

The Microfluidic Probe: Operation and Use for Localized Surface Processing
08:07

The Microfluidic Probe: Operation and Use for Localized Surface Processing

Published on: June 4, 2009

Rapid Subtractive Patterning of Live Cell Layers with a Microfluidic Probe
12:19

Rapid Subtractive Patterning of Live Cell Layers with a Microfluidic Probe

Published on: September 15, 2016

Fluorescence detection methods for microfluidic droplet platforms
14:16

Fluorescence detection methods for microfluidic droplet platforms

Published on: December 10, 2011

  • Hydrodynamic forces confine the microjet, enabling precise reagent delivery and aspiration.
  • The MFP can be scanned across a substrate surface for localized processing.

    • Main Results:

    • The MFP allows for precise, localized deposition and delivery of reagents.
    • The system enables scanning across large substrate areas for versatile applications.
    • Demonstrated assembly, mounting, alignment, and operation of the MFP for sample processing.

    Conclusions:

    • The microfluidic probe (MFP) provides a flexible and precise alternative for microscale fluid handling.
    • This technology enables dynamic, localized reagent delivery and surface processing.
    • The MFP facilitates advanced applications in cell biology and biotechnology research.