Related Experiment Video
Updated: Jun 21, 2026
FIBS-enabled Noninvasive Metabolic Profiling
Published on: February 3, 2014
Design and characterization of a maltose binding protein-linked growth factor for matrix engineering
Min Han1, In Su Park, Soo Hyun Kim
1Division of Life Sciences, Biomaterials Research Center, Korea Institute of Science and Technology, P.O. Box 131, Cheongryang, Seoul, 130-650, Korea.
Abstract:
The maltose-binding protein (MBP), which possesses a large number of exposed hydrophobic zones, can be used as a link for the immobilization of growth factors. The amount of immobilized MBP-vascular endothelial growth factors (VEGFs) for polystyrene surface was increased with respect to increasing protein, showing 1019 ng/cm(2) at 100 microg protein/ml. The phosphorylation of VEGF receptors in the MBP-VEGF stimulated HEK293/KDR cells as depicted from western blot analysis. Cell adhesion to a MBP-VEGF immobilized surface was mediated by the VEGF-VEGFR interaction. These results suggest that MBP-VEGFs are active and a MBP immobilization system can then anchor various bioactive proteins to hydrophobic surfaces.
