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A Guided Materials Screening Approach for Developing Quantitative Sol-gel Derived Protein Microarrays
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In situ assembly and screening of enzyme inhibitors with surface-tension microarrays

Laurent Mugherli1, Olga N Burchak, Larissa A Balakireva

  • 1Institut de Recherches en Technologies et Sciences pour le Vivant, CEA, 17 rue des Martyrs, 38054 Grenoble, France.

Angewandte Chemie (International Ed. in English)
|July 8, 2009
PubMed
Abstract

No abstract available in PubMed .

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In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.