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Co-immunoprecipitation Assay Using Endogenous Nuclear Proteins from Cells Cultured Under Hypoxic Conditions
09:17

Co-immunoprecipitation Assay Using Endogenous Nuclear Proteins from Cells Cultured Under Hypoxic Conditions

Published on: August 2, 2018

RNA-binding proteins implicated in the hypoxic response.

Kiyoshi Masuda1, Kotb Abdelmohsen, Myriam Gorospe

  • 1Laboratory of Cellular and Molecular Biology, National Institute on Aging-Intramural Research Program, National Institutes of Health, Baltimore, MD 21224, USA.

Journal of Cellular and Molecular Medicine
|July 9, 2009
PubMed
Summary

RNA-binding proteins (RBPs) control gene expression during low oxygen (hypoxia). Key RBPs like HuR and PTB regulate hypoxia-response genes, influencing cellular adaptation and disease.

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Analysis of Global RNA Synthesis at the Single Cell Level following Hypoxia

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Area of Science:

  • Molecular Biology
  • Cellular Stress Response

Background:

  • Gene expression is significantly altered by low oxygen (hypoxia).
  • Post-transcriptional modifications, particularly by RNA-binding proteins (RBPs), are crucial for regulating gene expression under stress.
  • RBPs play a pivotal role in modulating cellular responses to various stresses, including hypoxia.

Purpose of the Study:

  • To review the roles of RBPs in regulating mRNA turnover and translation during hypoxic conditions.
  • To highlight specific RBPs that influence the expression of hypoxia-response genes.
  • To discuss the therapeutic potential of targeting hypoxia-regulatory RBPs in disease.

Main Methods:

  • Literature review of studies investigating RNA-binding proteins and hypoxia.
  • Analysis of RBPs that interact with mRNAs encoding key hypoxia-response proteins.
  • Discussion of the mechanisms by which RBPs modulate gene expression under hypoxia.

Main Results:

  • HuR (human antigen R) and PTB (polypyrimidine tract-binding protein) associate with mRNAs like HIF-1alpha and VEGF, enhancing their expression post-hypoxia.
  • Other RBPs, including iron-response element-binding proteins (IRPs), cytoplasmic polyadenylation-element-binding proteins (CPEBs), and heterogeneous nuclear ribonucleoproteins (hnRNPs), also bind and regulate hypoxia-responsive transcripts.
  • These RBPs are essential for establishing and maintaining hypoxic gene expression patterns.

Conclusions:

  • RBPs are critical regulators of gene expression in response to hypoxia.
  • Targeting specific hypoxia-regulatory RBPs presents a promising therapeutic strategy for diseases characterized by aberrant hypoxic responses.