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Updated: Jun 21, 2026
Cell-Free DNA Extraction of Vitreous and Aqueous Humor Specimens for Diagnosis and Monitoring of Vitreoretinal Lymphoma
Published on: January 12, 2024
Cytokine and molecular analyses of intraocular lymphoma
Kouichi Ohta1, Kenji Sano, Hiroki Imai
1Department of Ophthalmology, Shinshu University School of Medicine, Matsumoto, Japan. ohta@po.mdu.ac.jp
Purpose:
The authors investigate the efficacy of using the cytokine levels and clonal heavy-chain immunoglobulin (IgH) gene rearrangements in the vitreous as adjunctive tools to diagnose intraocular lymphoma (IOL).
Methods:
The IL-10 and IL-6 levels and IgH gene rearrangements were analyzed in vitreous samples from 8 cases of IOL and in 14 uveitis patients.
Results:
The level of IL-10 with an IL-10/IL-6 ratio > 1 was significantly higher in all eyes with IOL. B-cell monoclonality was detected in only 5 of 8 eyes with IOL.
Conclusions:
The measurements of the levels of cytokines are valuable as a reliable biomarker.
