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Updated: Jun 21, 2026

Infinium Assay for Large-scale SNP Genotyping Applications
Published on: November 19, 2013
Use of SNP-arrays for ChIP assays: computational aspects
Enrique M Muro1, Jennifer A McCann, Michael A Rudnicki
1Max-Delbrück Center for Molecular Medicine, Berlin, Germany.
Abstract:
The simultaneous genotyping of thousands of single nucleotide polymorphisms (SNPs) in a genome using SNP-Arrays is a very important tool that is revolutionizing genetics and molecular biology. We expanded the utility of this technique by using it following chromatin immunoprecipitation (ChIP) to assess the multiple genomic locations protected by a protein complex recognized by an antibody. The power of this technique is illustrated through an analysis of the changes in histone H4 acetylation, a marker of open chromatin and transcriptionally active genomic regions, which occur during differentiation of human myoblasts into myotubes. The findings have been validated by the observation of a significant correlation between the detected histone modifications and the expression of the nearby genes, as measured by DNA expression microarrays. This chapter focuses on the computational analysis of the data.
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