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Updated: Jun 21, 2026

Stability and Structure of Bat Major Histocompatibility Complex Class I with Heterologous β2-Microglobulin
Published on: March 10, 2021
The intracellular localization and oligomerization of chicken invariant chain with major histocompatibility complex
Abstract:
Invariant chain (Ii) binds to MHC class II (MHCII) to assemble a nonamer in the endoplasmic reticulum. Major histocompatibility complex class II-associated Ii peptide (CLIP) that occupies the peptide binding groove of MHCII prevents MHCII molecules from loading with endogenous antigens. We used the green or red fluorescent protein-fused Ii or MHCII subunits to detect the intracellular localization and oligomerization of chicken Ii with single chicken MHCII subunits. Our results indicated that chicken Ii associates with single MHCII subunits and formed oligomers with MHCII subunits. The Ii mutant with a deleted CLIP sequence blocks the association with single MHCII subunits, but exchanging CLIP with the Newcastle disease virus F(343) epitope restores this association. Thus, MHCII polymer assembly is not blocked as long as the basic steric molecular structure of Ii is maintained, and different binding models exist in different species or MHCII isotypes.
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