Related Experiment Video
Updated: Jun 21, 2026

Bacteriophage Effectiveness for Biocontrol of Foodborne Pathogens Evaluated via High-Throughput Settings
Published on: August 19, 2021
Isolation and characterization of virulent bacteriophages against Escherichia coli serogroups O1, O2, and O78
N Jamalludeen1, Y-M She, E J Lingohr
1Department of Food Science, University of Guelph, Guelph, Ontario N1G 3W1, Canada.
Abstract:
The goal of this study was to isolate and characterize a more complete set of phages that are active against Escherichia coli serogroups O1, O2, and O78, the main causative agents of avian colibacillosis. A mixture of E. coli (O1:K1), (O2:K1), and (O78:K80) was used as a host to isolate phages from wastewater and fecal samples from poultry processing plants. Seven phages were isolated; only 2 of them, EC-Nid1 and EC-Nid2, were selected for further characterization. It was found that EC-Nid1 and EC-Nid2 had icosahedral heads, necks, and contractile tails with tail fibers and therefore belonged to Myoviridae. The phages had genome sizes of 67.06 to 68.04 kb and they lysed all tested strains of E. coli serotype O1, O2, and O78. The 2 phages were resistant to pH 5 to 9, and phage EC-Nid2 was slightly more resistant to acid and alkali environments. Two major protein bands were indicated in EC-Nid (A and D); band D at 45 kDa was a major coat protein and band A was identified as a homolog of endo-N-acetylneuraminidase. It was concluded that phage EC-Nid1 and EC-Nid2 are highly active against O1, O2, and O78 colibacillosis strains.
More Related Videos
09:44Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing
Published on: December 23, 2022
08:46T4 Bacteriophage and E. coli Interaction in the Murine Intestine: A Prototypical Model for Studying Host-Bacteriophage Dynamics In Vivo
Published on: January 26, 2024
Related Concept Videos
Viral Replication: Lytic Cycle
Bacterial Gastroenteritis
Lysogenic Cycle of Bacteriophages
Regulation of Bacterial Virulence
Lytic Cycle of Bacteriophages
Bacteriophages of the Human Virome