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Updated: Jun 21, 2026

Application of Laser Micro-irradiation for Examination of Single and Double Strand Break Repair in Mammalian Cells
Published on: September 5, 2017
Utilization of salmon milt DNA against UV damage
Yoshiharu Sasaki1, Daisuke Miyoshi, Naoki Sugimoto
1FIBER (Frontier Institute for Biomolecular Engineering Research), Konan University, 7-1-20 Minatojima-minamimachi, Chuo-ku, Kobe, 650-0047, Japan.
Abstract:
We examined the effect of ultraviolet (UV) irradiation on the UV spectra and radical scavenging activity of DNA strands and found that the absorption spectra of salmon milt DNA was extended up to about 350 nm after ultraviolet C (UVC, 100-280 nm) irradiation with 300 kJ/m(2). The UV B (UVB, 280-315 nm) protection ability of UVC-irradiated salmon milt DNA for a single-stranded target DNA (19-mer) was further studied. The percentage of damaged target DNA after 50 kJ/m(2) of UVB irradiation in the presence of UVC-irradiated salmon milt DNA, UVC-unirradiated salmon milt DNA, and 2-phenylbenzimidazole sulfonic acid was estimated to be 24.6%, 27.0%, and 18.9%, respectively. Moreover, the ultraviolet A (UVA, 315-400 nm)/UVB ratio and critical wavelength of natural (UVC-unirradiated) salmon milt DNA were estimated to be 0.13 and 313 nm, respectively, whereas those of the UVC-irradiated salmon milt DNA were 0.34 and 375 nm, respectively. Interestingly, the value of 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity in UVC-irradiated salmon milt DNA was about five times higher than that of UVC-unirradiated salmon milt DNA. These results indicate that the UVC-irradiated salmon milt DNA could be useful as a protector against a wide range of UV light from UVC approximately UVA.
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