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Updated: Jun 21, 2026
In Vivo Microinjection and Electroporation of Mouse Testis
Published on: August 23, 2014
[Expression of high mobility group protein A in male mouse testicular cell lines]
Zheng-bing Ma1, Ying Yang, Jun Wang
1Department of Obstetrics and Gynecology, Tangdu Hospital, The Fourth Military Medical University, Xi'an, Shaanxi 710038, China.
Objective:
To detect the expression of high mobility group protein A (HMGA) in male mouse testicular cell lines TM4, GC-1spg and GC-2spd(ts), and to pave the theoretical ground for further investigation of the action mechanism of the HMGA gene in male mouse spermatogenesis.
Methods:
We detected the expressions of HMGA1 and HMGA2 by RT-PCR and Western blot in the male mouse testicular cell lines TM4, GC-1spg and GC-2spd(ts).
Results:
HMGA1 and HMGA2 were expressed in the male mouse testicular cell lines TM4, GC-1spg and GC-2spd(ts) at both mRNA and protein levels. Western blot and RT-PCR methods showed similar results.
Conclusion:
The expression of HMGA may be involved in the cell division and proliferation of TM4, GC-1spg and GC-2spd(ts) and play an important role in spermatogenesis of male mice.
