Mouse embryo cryopreservation utilizing a novel high-capacity vitrification spatula

Area of Science:

• Reproductive Biology
• Cryobiology
• Genetics

Background:

• Embryo cryopreservation is crucial for reproductive programs and maintaining genetically modified mouse strains.
• Current methods can be limited in capacity and efficiency.

Purpose of the Study:

• To introduce a novel, homemade vitrification spatula (VS) for efficient cryopreservation of preimplantation mammalian embryos.
• To evaluate the survival rates and fertility of embryos vitrified using the VS.

Main Methods:

• Development of a readily homemade vitrification spatula (VS) with a large holding capacity.
• Vitrification of preimplantation mammalian embryos (one-cell stage and morulae) in micro-drops using ultra-rapid cooling in liquid nitrogen (LN2).
• Assessment of post-thaw survival rates and fertility of the derived progeny.

Main Results:

• Vitrified one-cell embryos and morulae exhibited high survival rates after thawing.
• Fertility of offspring derived from vitrified embryos was comparable to the control group.
• The VS demonstrated a large holding capacity (up to 50 embryos), facilitating rapid expansion of storage.

Conclusions:

• The homemade vitrification spatula provides an effective and efficient method for mammalian embryo cryopreservation.
• This technique enhances storage capacity and streamlines the generation of transgenic mice.
• The VS is a valuable tool for reproductive programs and animal facilities utilizing genetically modified models.