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Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.
RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
Genomics02:02

Genomics

Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
Sanger Sequencing01:57

Sanger Sequencing

DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...
Genome Annotation and Assembly03:36

Genome Annotation and Assembly

The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.

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Related Experiment Video

Updated: Jun 21, 2026

Sequencing of mRNA from Whole Blood using Nanopore Sequencing
11:26

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Published on: June 3, 2019

High-throughput next-generation sequencing technologies foster new cutting-edge computing techniques in

Mary Qu Yang1, Brian D Athey, Hamid R Arabnia

  • 1National Human Genome Research Institute, National Institutes of Health (NIH), U.S. Department of Health and Human Services, Bethesda, MD 20892, USA. yangma@mail.NIH.gov

BMC Genomics
|July 15, 2009
PubMed
Summary

High-throughput sequencing drives supercomputing in genomics, personalized medicine, and bioinformatics. This conference successfully fostered interdisciplinary research, selecting 19 high-quality papers for publication.

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Last Updated: Jun 21, 2026

Sequencing of mRNA from Whole Blood using Nanopore Sequencing
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Published on: June 3, 2019

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Targeted Next-generation Sequencing and Bioinformatics Pipeline to Evaluate Genetic Determinants of Constitutional Disease

Published on: April 4, 2018

Area of Science:

  • Bioinformatics and Computational Biology
  • Genomics
  • Personalized Medicine

Background:

  • High-throughput next-generation sequencing (NGS) enables advanced applications in genomics and personalized medicine.
  • Supercomputing plays a crucial role in analyzing large-scale biological data.
  • The 2008 International Conference on Bioinformatics and Computational Biology (Biocomp) aimed to advance interdisciplinary research.

Purpose of the Study:

  • To promote synergistic inter/multidisciplinary research and education in bioinformatics and computational biology.
  • To address current research trends and advances in areas like genome sequencing and personalized medicine.
  • To select and publish high-quality research papers presented at the conference.

Main Methods:

  • A large international conference (Biocomp 2008) was held, attracting over two thousand participants.
  • Thousands of research papers were submitted and underwent rigorous peer review by at least three reviewers.
  • A select 19 high-quality papers were chosen for publication in a BMC Genomics supplement based solely on peer review.

Main Results:

  • The conference successfully fostered interdisciplinary collaboration and knowledge exchange.
  • A highly selective process resulted in the identification of 19 top-tier research papers.
  • Keynote lectures were delivered by prominent figures in science and technology.

Conclusions:

  • The conference achieved its goal of promoting synergistic research and education in bioinformatics.
  • The selected papers represent significant advancements in high-throughput sequencing applications.
  • The event highlighted the growing importance of computational approaches in biological sciences.