Chromosome-scale reference genome assembly and annotation of Prunus scopulorum

Area of Science:

• Genomics
• Plant Science
• Evolutionary Biology

Background:

• Prunus scopulorum is a valuable Chinese wild cherry germplasm resource, known for its cold tolerance and adaptability.
• Understanding its genetic makeup is crucial for its conservation and utilization.

Purpose of the Study:

• To construct a high-quality, chromosome-level whole-genome map of Prunus scopulorum.
• To provide a foundational resource for future research on this species.

Main Methods:

• Whole-genome sequencing and assembly.
• Identification and analysis of repetitive sequences.
• Gene prediction and functional annotation.

Main Results:

• A 248.6 Mb genome assembly with a scaffold N50 of 27.83 Mb was generated.
• Repetitive sequences constitute 44.96% of the genome, including LINEs, LTRs, and SINEs.
• 32,542 protein-coding genes were predicted, with 99.41% functional annotation; BUSCOs value reached 97.0% and LAI reached 17.04.

Conclusions:

• The high-quality genome assembly of Prunus scopulorum is a significant resource.
• This assembly will facilitate studies on the evolution within the Prunus genus.
• It will also support research into important agronomic traits of P. scopulorum.