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Published on: August 22, 2018
[Sample preprocessing method for residual quinolones in honey using immunoaffinity resin]
Yoshiharu Ihara1, Mihoko Kato, Tsukasa Kodaira
1Frontier Institute Co., Ltd, Ishikari, Hokkaido 0613241, Japan.
Summary
A new immunoaffinity resin method effectively detects quinolone residues in honey. This technique offers high recovery rates and low quantification limits for accurate honey safety testing.
Area of Science:
- Food Chemistry
- Analytical Chemistry
- Immunochemistry
Context:
- Quinolone antibiotics are widely used in animal husbandry.
- Residues of quinolones can potentially contaminate food products like honey.
- Accurate detection methods are crucial for ensuring food safety and regulatory compliance.
Purpose:
- To develop and validate an immunoaffinity resin-based sample preparation method for determining quinolone residues in honey.
- To assess the efficiency, recovery, and quantification limits of the developed method.
Summary:
- An immunoaffinity resin was prepared by immobilizing a quinolone-specific monoclonal antibody onto agarose.
- Honey samples were processed by diluting, reacting with the resin, washing, and eluting quinolones with glycine-HCl.
- Quinolones in the eluate were quantified using High-Performance Liquid Chromatography (HPLC) with fluorescence detection, showing no interfering peaks.
Impact:
- The developed method demonstrates high recoveries (>70%) for various quinolones at low fortification levels (10-20 ng/g).
- The quantification limit of 2 ng/g is suitable for monitoring quinolone residues in honey.
- This immunoaffinity resin method is effective and practical for routine analysis of residual quinolones in honey, enhancing food safety monitoring.
