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Updated: Jun 21, 2026

Optimized Bone Sampling Protocols for the Retrieval of Ancient DNA from Archaeological Remains
Published on: November 30, 2021
Identification of a disinterred grave by molecular and stable isotope analysis
Ian D Bull1, Robert Berstan, Arpad Vass
1Organic Geochemistry Unit, Bristol Biogeochemistry Research Centre, School of Chemistry, University of Bristol, Cantock's Close, Bristol, BS8 1TS, UK. ian.d.bull@bris.ac.uk
Abstract:
Confirmation of a potential disinterred grave was sought by GC and GC/MS analyses of lipid extracts of whole soils and white particulate matter. Fatty acid profiles and concentrations determined for three of the soils correlated with the deposition of a large amount of exogenous organic matter, most likely adipocere and/or decomposed body fat. Determination of C16:0 and C18:0 fatty acid delta13C values by GC/C/IRMS revealed the input to be isotopically distinct from common British domesticated animals, plotting closely to values determined for adipose fat obtained from of a murder victim. By considering the difference between delta13C values (delta13C18:0-16:0) a potential isotopic proxy for identifying the source of adipocere (human) and adipose tissue was proposed.

