Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Formation of the Platelet Plug

Formation of the Platelet Plug

The platelet phase, the second stage of hemostasis, commences around 15-20 seconds after an injury. It follows and overlaps with the vascular phase, during which blood vessels constrict to minimize blood loss.
As the injured blood vessel contracts, endothelial cells undergo contraction, revealing collagen fibers in the basement membrane and underlying connective tissue. Furthermore, the plasma membrane of endothelial cells becomes adhesive, preparing the site for platelet adhesion. Platelets...

Structure and Function of Platelets

Structure and Function of Platelets

The cell fragments known as platelets are disc-shaped, with an average diameter of about 3 μm and a thickness of roughly 1 μm. They play a crucial role in the body's vascular clotting system, which also involves plasma proteins, blood cells, and blood vessel tissues.
Platelets are continually replenished, circulating in the bloodstream for 9-12 days before being removed by phagocytes, primarily in the spleen. A microliter of circulating blood contains between 150,000 and 450,000 platelets, with...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Targeting Diabetic Retinopathy with Human iPSC-Derived Vascular Reparative Cells in a Type 2 Diabetes Model.

Cells·2025

Same author

Direct in vivo reprogramming to relieve tissue ischemia via induced vasculogenesis.

Molecular therapy : the journal of the American Society of Gene Therapy·2025

Same author

Tissue nanotransfection-based endothelial PLCγ2-targeted epigenetic gene editing rescues perfusion and diabetic ischemic wound healing.

Molecular therapy : the journal of the American Society of Gene Therapy·2025

Same author

Fully biologic endothelialized-tissue-engineered vascular conduits provide antithrombotic function and graft patency.

Cell stem cell·2024

Same author

Vasculogenic skin reprogramming requires TET-mediated gene demethylation in fibroblasts for rescuing impaired perfusion in diabetes.

Nature communications·2024

Same author

Thrombopoietic agents enhance bone healing in mice, rats, and pigs.

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research·2024

Search research articles

Related Experiment Video

Updated: Jun 21, 2026

Analyzing Platelet Subpopulations by Multi-color Flow Cytometry

Analyzing Platelet Subpopulations by Multi-color Flow Cytometry

Published on: June 10, 2025

Platelet MPs obscure some EPC definitions.

Mervin C Yoder¹

¹Indiana University, IN, USA.

|July 18, 2009

Summary

Contaminating platelet microparticles (MPs) can be mistakenly identified as endothelial progenitor cells (EPCs) in vitro assays. This uptake leads to significant misinterpretation of cell surface phenotype results.

Area of Science:

Cell biology
Hematology
Immunology

Background:

Endothelial progenitor cells (EPCs) are crucial for vascular repair and are typically identified by specific cell surface markers in vitro.
Commonly used in vitro assays for EPC identification may be susceptible to confounding factors.
Platelet-derived microparticles (MPs) are small vesicles released from platelets, known to interact with other cell types.

Discussion:

The study investigates a potential artifact in EPC identification assays.
It proposes that the observed cell surface phenotype of EPCs might be due to the uptake of platelet MPs by mononuclear cells.
This phenomenon could lead to a gross misinterpretation of the assay's results, overestimating EPC populations.

Key Insights:

The cell surface phenotype defining EPCs in a common in vitro assay may not represent true EPCs.

More Related Videos

Comprehensive Analysis of Procoagulant Platelets Exhibiting Features of Necrosis, Apoptosis and Platelet Activation

Comprehensive Analysis of Procoagulant Platelets Exhibiting Features of Necrosis, Apoptosis and Platelet Activation

Published on: May 23, 2025

Isolation of Endothelial Progenitor Cells from Healthy Volunteers and Their Migratory Potential Influenced by Serum Samples After Cardiac Surgery

Isolation of Endothelial Progenitor Cells from Healthy Volunteers and Their Migratory Potential Influenced by Serum Samples After Cardiac Surgery

Published on: February 14, 2017

Related Experiment Videos

Last Updated: Jun 21, 2026

Analyzing Platelet Subpopulations by Multi-color Flow Cytometry

Analyzing Platelet Subpopulations by Multi-color Flow Cytometry

Published on: June 10, 2025

Comprehensive Analysis of Procoagulant Platelets Exhibiting Features of Necrosis, Apoptosis and Platelet Activation

Comprehensive Analysis of Procoagulant Platelets Exhibiting Features of Necrosis, Apoptosis and Platelet Activation

Published on: May 23, 2025

Isolation of Endothelial Progenitor Cells from Healthy Volunteers and Their Migratory Potential Influenced by Serum Samples After Cardiac Surgery

Isolation of Endothelial Progenitor Cells from Healthy Volunteers and Their Migratory Potential Influenced by Serum Samples After Cardiac Surgery

Published on: February 14, 2017

Uptake of contaminating platelet MPs by cultured mononuclear cells is identified as a likely source of this artifact.

This finding highlights a critical limitation in current EPC characterization methods.

Outlook:

Re-evaluation of current in vitro EPC identification protocols is necessary.
Development of more specific assays to distinguish true EPCs from MP-associated artifacts is recommended.
Further research should focus on the precise mechanisms of MP-EPC interaction and its physiological implications.