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Updated: Jun 21, 2026

Live-cell Imaging of Lysosomal Membrane Permeabilization During Necroptosis
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Live to dead cell imaging.

Stephen W G Tait1, Lisa Bouchier-Hayes, Andrew Oberst

  • 1Department of Immunology, St Jude Children's Research Hospital, Memphis, TN, USA.

Methods in Molecular Biology (Clifton, N.J.)
|July 18, 2009
PubMed
Summary
This summary is machine-generated.

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Live cell imaging enables visualization of apoptosis markers like mitochondrial outer membrane permeabilization (MOMP) and phosphatidylserine exposure. This protocol details tracking multiple apoptotic events in single cells over time for kinetic analysis.

Area of Science:

  • Cell Biology
  • Biochemistry
  • Molecular Biology

Background:

  • Live cell imaging offers a dynamic approach to studying cellular processes.
  • Apoptosis involves a series of complex events, including mitochondrial outer membrane permeabilization (MOMP).
  • Visualizing multiple apoptotic markers simultaneously provides deeper insights into cell death pathways.

Purpose of the Study:

  • To describe a protocol for live cell imaging of multiple apoptotic events.
  • To enable simultaneous visualization of key indicators of apoptosis in single cells.
  • To facilitate quantitative kinetic analysis of apoptosis progression.

Main Methods:

  • Generation of a stable cell line expressing fluorescent apoptotic markers (e.g., cytochrome c-GFP).
  • Utilizing a combination of fluorescent fusion proteins and probes to label diverse apoptotic events.

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Last Updated: Jun 21, 2026

Live-cell Imaging of Lysosomal Membrane Permeabilization During Necroptosis
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Live-cell Imaging of Lysosomal Membrane Permeabilization During Necroptosis

Published on: November 14, 2025

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06:06

Studying Cell Death Initiation Using a Digital Microscope

Published on: November 10, 2023

Long-term Live-cell Imaging to Assess Cell Fate in Response to Paclitaxel
08:29

Long-term Live-cell Imaging to Assess Cell Fate in Response to Paclitaxel

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  • Inducing apoptosis in cells and performing continuous live cell imaging.
  • Conducting post-imaging quantitative kinetic analysis of observed apoptotic processes.

    • Main Results:

    • Demonstration of a protocol to image multiple apoptotic events in the same cell over time.
    • Successful visualization of events such as MOMP, mitochondrial dysfunction, phosphatidylserine exposure, and membrane permeabilization.
    • Establishment of a method for quantitative kinetic analysis of apoptosis progression.

    Conclusions:

    • The described protocol allows for comprehensive, time-resolved visualization of apoptosis.
    • This method enhances the understanding of the kinetics and interplay of multiple apoptotic events.
    • The technique is valuable for studying cell death mechanisms in various biological contexts.