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Updated: Jun 21, 2026

Radiolabeling and Quantification of Cellular Levels of Phosphoinositides by High Performance Liquid Chromatography-coupled Flow Scintillation
10:52

Radiolabeling and Quantification of Cellular Levels of Phosphoinositides by High Performance Liquid Chromatography-coupled Flow Scintillation

Published on: January 6, 2016

Form and flexibility in phosphoinositide 3-kinases

Roger Williams1, Alex Berndt, Simon Miller

  • 1MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 0QH, UK. rlw@mrc-lmb.cam.ac.uk

Biochemical Society Transactions
|July 21, 2009
PubMed

Insights

Phosphoinositide 3-kinases (PI3Ks) are crucial for cell survival and growth, with mutations linked to cancer. Understanding PI3K structure aids in developing targeted cancer therapies and cell biology tools.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cancer Research

Background:

  • Phosphoinositide 3-kinases (PI3Ks) regulate essential cellular processes like survival, proliferation, and migration.
  • Gain-of-function mutations in PI3K genes are frequently observed in various cancers, highlighting their role in tumorigenesis.

Purpose of the Study:

  • To elucidate the mechanistic basis of PI3K's role in cancer development through ongoing structural studies.
  • To identify and develop isotype-specific PI3K inhibitors for applications in cell biology and therapeutics.

Main Methods:

  • Structural studies of PI3K enzymes and their regulatory subunit complexes.
  • Analysis of PI3K's catalytic cycle and enzyme flexibility.

Main Results:

  • Structural insights are clarifying the mechanistic underpinnings of PI3K's involvement in tumor development.
  • The broad biological activities of PI3K isotypes necessitate the search for specific inhibitors.

Conclusions:

  • Structural studies of PI3Ks are pivotal for understanding their oncogenic roles.
  • Developing isotype-specific PI3K inhibitors is a key strategy for both research tools and cancer therapy.

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