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Influence of reduction in the elution times on HPLC glycohaemoglobin results
Antonio Leon-Justel1, Jose D Santotoribio, Inmaculada Dominguez-Pascual
1Department of Clinical Biochemistry, Laboratory Buildings (4th Floor), Virgen del Rocío University Hospital, IBIS/CSIC/University of Seville, Seville, Spain. antonio.leonj.sspa@juntadeandalucia.es
Objectives:
To compare HPLC methods with short and long elution times for HbA1c measurement in blood.
Methods:
Comparison between G7-Tosoh (1.2 min); Bio-Rad-Variant-II-Turbo (1.3 min) and Arkray-HA-8160 (2.9 min).
Results:
Passing-Bablok regression equations were: Y=0.17+0.96X; Y=-0.39+1.01X; Y=-0.40+1.0X and the means of the differences using Bland-Altman Plot were 0.02; -0.34; 0.32 for HA-8160/G7-Tosoh, HA-8160/Variant-II-Turbo and G7-Tosoh/Variant-II-Turbo, respectively.
Conclusions:
Faster elution methods had no problems on reproducibility of results obtained by slower elution methods.
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