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Related Experiment Video

Updated: Jun 21, 2026

A Microfluidic Platform for High-throughput Single-cell Isolation and Culture
09:51

A Microfluidic Platform for High-throughput Single-cell Isolation and Culture

Published on: June 16, 2016

Droplet microfluidic technology for single-cell high-throughput screening.

Eric Brouzes1, Martina Medkova, Neal Savenelli

  • 1RainDance Technologies, Lexington, MA 02421, USA. brouzese@raindancetech.com

Proceedings of the National Academy of Sciences of the United States of America
|July 21, 2009
PubMed
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We developed a microfluidic droplet technology for high-throughput single mammalian cell screening. This platform enables rapid cytotoxicity screening and analysis of cell viability within droplets.

Area of Science:

  • Biotechnology
  • Microfluidics
  • Cell Biology

Background:

  • High-throughput screening of single cells is crucial for drug discovery and biological research.
  • Existing methods often face limitations in throughput, scalability, and precision.
  • Microfluidic technologies offer potential solutions for precise cellular manipulation and analysis.

Purpose of the Study:

  • To present and validate a droplet-based microfluidic platform for high-throughput single mammalian cell screening.
  • To develop and assess a droplet viability assay for quantitative cell analysis.
  • To demonstrate the utility of the platform in a cytotoxicity drug screening application.

Main Methods:

  • Encapsulation of single cells and reagents in microdroplets (1 pL to 10 nL) within an immiscible carrier oil.

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Microfluidic Approach to Resolve Simultaneous and Sequential Cytokine Secretion of Individual Polyfunctional Cells
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Microfluidic Approach to Resolve Simultaneous and Sequential Cytokine Secretion of Individual Polyfunctional Cells

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Last Updated: Jun 21, 2026

A Microfluidic Platform for High-throughput Single-cell Isolation and Culture
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Published on: June 16, 2016

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Published on: March 8, 2024

  • Development of a droplet viability assay for quantitative scoring of cell viability and growth.
  • Utilization of an optically-coded droplet library for identification of droplet composition.
  • Screening of a drug library for cytotoxic effects on human monocytic U937 cells.

    • Main Results:

    • Demonstrated high viability of encapsulated U937 cells over 4 days.
    • Successfully validated a droplet-based cytotoxicity screening workflow.
    • Quantitatively scored cell viability and growth within intact droplets.
    • Identified cytotoxic effects of a drug library on U937 cells using the microfluidic platform.

    Conclusions:

    • The developed droplet microfluidic platform is modular, robust, and enables high-throughput single-cell analysis.
    • The technology facilitates combinatorial screening and analysis of small sample volumes.
    • This platform offers a versatile tool for various applications in cell biology and drug discovery.