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Repeatability of exercise behaviors in mice.

Amy M Knab1, Robert S Bowen, Trudy Moore-Harrison

  • 1Department of Kinesiology, University of North Carolina, Charlotte, United States. knabam@appstate.edu

Physiology & Behavior
|July 22, 2009
PubMed
Summary

Maximal treadmill endurance tests in mice show poor repeatability due to high intra-mouse variability. However, voluntary wheel running (WR) measurements demonstrate high consistency and stability within individual mice, indicating reliable assessment of physical activity.

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Area of Science:

  • Exercise physiology
  • Animal models
  • Behavioral neuroscience

Background:

  • Rodent exercise models are crucial for studying physiological and behavioral responses.
  • Repeatability data for common exercise measurements in mice are limited.
  • Understanding measurement reliability is essential for accurate research findings.

Purpose of the Study:

  • To assess the repeatability of maximal treadmill endurance tests in mice.
  • To evaluate the repeatability of voluntary wheel running (WR) for physical activity assessment in mice.
  • To compare the reliability of two common methods for measuring exercise behavior in mice.

Main Methods:

  • Repeatability of treadmill tests analyzed in inbred and outbred mice.
  • A 10-week repeatability analysis was conducted using Balb/cJ mice (n=20).

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  • Voluntary physical activity (WR) was measured by distance, duration, and speed; WR measurements on days 5 and 6 in a large cohort (n=739) were compared.

    • Main Results:

    • Treadmill endurance tests showed significant intra-mouse variability and lack of agreement/correlation within mice.
    • No significant differences were found for wheel running (WR) measurements within mice between days (p=0.99).
    • High correlations (r=0.74-0.85) were observed between days for WR measurements within mice.

    Conclusions:

    • Maximal treadmill endurance testing in mice exhibits high intra-mouse variability, suggesting poor repeatability.
    • Voluntary wheel running (WR) measurements show high correlation and agreement, indicating repeatable and stable assessment of physical activity.
    • Wheel running is a more reliable method for assessing spontaneous physical activity in mice compared to treadmill endurance tests.