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How to Stabilize Protein: Stability Screens for Thermal Shift Assays and Nano Differential Scanning Fluorimetry in the Virus-X Project
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Published on: February 11, 2019

Enzyme stabilization by domain insertion into a thermophilic protein

Chung-Sei Kim1, Brennal Pierre, Marc Ostermeier

  • 1Othmer-Jacobs Department of Chemical and Biological Engineering, Polytechnic Institute of New York University, 6 MetroTech Center, Brooklyn, NY 11201, USA.

Protein Engineering, Design & Selection : PEDS
|July 23, 2009
PubMed
Summary

Researchers stabilized exoinulinase (EI) by inserting its domain into a scaffold protein. This novel method enhances enzyme kinetic stability without altering its activity, opening new applications in inulin processing and bioethanol production.

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