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Visualizing Early Infection Sites of Rice Blast Disease (Magnaporthe oryzae) on Barley (Hordeum vulgare) Using a Basic Microscope and a Smartphone
Published on: March 17, 2023
Pedobacter oryzae sp. nov., isolated from rice paddy soil
Yeji Jeon1, Jeong Myeong Kim, Jin Ho Park
1Environmental Biotechnology National Core Research Center, Gyeongsang National University, Jinju 660-701, Republic of Korea.
Abstract:
A Gram-stain-negative, strictly aerobic bacterium, designated strain N7(T), was isolated from a rice paddy in South Korea. Cells of strain N7(T) were non-motile, non-spore-forming rods. Growth was observed at 15-35 degrees C (optimum of 25-30 degrees C) and between pH 6.0 and 8.0 (optimum of pH 6.5-7.5). The predominant isoprenoid quinone was menaquinone-7. The major cellular fatty acids of strain N7(T) were summed feature 3 (comprising C(16 : 1)omega7c and/or iso-C(15 : 0) 2-OH), iso-C(15 : 0), anteiso-C(15 : 0), C(15 : 0) and iso-C(16 : 0). The G+C content of the genomic DNA was 37.7 mol%. Comparative 16S rRNA gene sequence analyses showed that strain N7(T) formed a distinct phyletic line within the genus Pedobacter. Phylogenetic distances from strains of other Pedobacter species with validly published names were greater than 5.0 % (i.e. <95.0 % 16S rRNA gene sequence similarities). On the basis of phenotypic and molecular data, it is clear that strain N7(T) represents a novel species within the genus Pedobacter, for which the name Pedobacter oryzae sp. nov. is proposed. The type strain is N7(T) (=KACC 12821(T) =DSM 19973(T)).
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