[Comparison on the HPLC fingerprint of Cassia obtusifolia between the raw and roasted seeds]
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Guiliu Li1, Yongqing Xiao, Cun Zhang
1Institute of Chinese Materia Madica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
Objective:
To establish HPLC fingerprint for the identification of Cassia obtusifolia and compare chemical constituents of the raw and roasted seeds of C. obtusifolia.
Method:
Chromatographic fingerprint of C. obtusifolia was investigated by RP-HPLC and the gradient elution mode was applied to chromatographic separation. Data were analyzed by fingerprint similarity evaluation software to compare the similarity of the samples for identifying the main chromatographic peaks furthermore.
Result:
Having compared the HPLC fingerprint of the raw and roasted seeds of C. obtusifolia were compared preliminarily, and 12 main chromatographic peaks were identified.
Conclusion:
HPLC method can be used in quality control and identification of the raw and roasted seeds of C. obtusifolia.
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