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Optimization of preservatives in a topical formulation using experimental design
Y Rahali1, A-M Pensé-Lhéritier, C Mielcarek
1Laboratoire de Pharmacie Galénique et Pharmacotechnie Industrielle, Faculté de Médecine et de Pharmacie de Rabat, 6203, Université Mohammed V - Souissi Maroc. younesrahali@gmail.com
Abstract:
Optimizing the preservative regime for a preparation requires the antimicrobial effectiveness of several preservative combinations to be determined. In this study, three preservatives were tested: benzoic acid, sorbic acid and benzylic alcohol. Their preservative effects were evaluated using the antimicrobial preservative efficacy test (challenge-test) of the European Pharmacopeia (EP). A D-optimal mixture design was used to provide a maximum of information from a limited number of experiments. The results of this study were analysed with the help of the Design Expert software and enabled us to formulate emulsions satisfying both requirements A and B of the EP.
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