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Updated: Jun 21, 2026

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Simultaneous DNA-RNA Extraction from Coastal Sediments and Quantification of 16S rRNA Genes and Transcripts by Real-time PCR
Published on: June 11, 2016
Sequence specific primer extension RNA analysis (SeSPERA) for the investigation of substrate utilization of microbial
Alessandro G Franchini1, Marcell Nikolausz, Matthias Kästner
1Department of Environmental Biotechnology, UFZ, Helmholtz Centre for Environmental Research, Leipzig, Germany.
Journal of Microbiological Methods
|July 25, 2009
Abstract:
A simple, fast and cost-effective method for the taxon-specific separation of various 16S rRNAs was developed. This primer extension mediated separation of different RNA species was obtained on agarose gels. The method provides an easy way for the investigation of substrate mediated radioisotope incorporation into the RNA.
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