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Published on: May 28, 2019
Attenuation of lipopolysaccharide-mediated left ventricular dysfunction by glutamine preconditioning
Gang Chen1, Tom G Neilan, Hong Chen
1Department of Surgery, Royal College of Surgeons in Ireland, Education and Research CentreBeaumont Hospital, Dublin 9, Ireland. gchen@rcsi.ie
The Journal of Surgical Research
|July 25, 2009
Summary
Glutamine pretreatment protects the heart from inflammation-induced dysfunction by increasing heat shock protein 72 (HSP72) expression. This suggests glutamine can activate cardiac preconditioning responses for clinical use.
Area of Science:
- Cardiology
- Biochemistry
- Pharmacology
Background:
- Myocardial dysfunction commonly occurs post-surgery due to inflammatory responses.
- Lipopolysaccharide (LPS) is a key trigger of this inflammatory response and subsequent cardiac dysfunction.
- Preconditioning strategies aim to protect the heart against such insults.
Purpose of the Study:
- To investigate the cardioprotective effects of glutamine pretreatment.
- To determine if glutamine acts as a preconditioning agent against LPS-induced myocardial dysfunction.
- To explore the role of heat shock protein 72 (HSP72) in glutamine-mediated cardioprotection.
Main Methods:
- Mice were divided into four groups: control, LPS, glutamine + LPS, and Quercetin (HSP inhibitor) + glutamine + LPS.
- Left ventricular function was assessed using invasive hemodynamics 6 hours post-LPS.
- HSP72 expression in heart tissue was measured via Western immunoblot 12 hours after glutamine administration.
Main Results:
- LPS significantly impaired left ventricular function, decreasing LVESP and dP/dtmax, and increasing LVEDP.
- Glutamine pretreatment 12 hours before LPS induced HSP72 expression and significantly attenuated LPS-induced cardiac dysfunction.
- Quercetin partially blocked HSP72 induction and abolished the protective effects of glutamine.
Conclusions:
- Glutamine pretreatment confers cardioprotection against LPS-induced myocardial dysfunction.
- This cardioprotection is linked to the induction of HSP72.
- Glutamine may represent a viable clinical strategy for activating cardiac preconditioning.
