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Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
The Unfolded Protein Response01:37

The Unfolded Protein Response

The ER is the hub of protein synthesis in a cell. It has robust systems to quality control protein folding and also for degradation of terminally misfolded proteins. Under normal conditions, a small proportion of misfolded proteins that cannot be salvaged need to be transported to the cytoplasm by the ER-associated degradation or ERAD pathways. However, if the ERAD cannot handle the misfolded proteins, the cell activates the unfolded protein response or UPR to adjust the protein folding...
Constitutive and Regulated Gene Expression01:27

Constitutive and Regulated Gene Expression

Gene expression in prokaryotes is governed by constitutive and regulated systems, allowing cells to balance the production of essential proteins with adaptive responses to environmental changes.Constitutive Gene ExpressionConstitutive, or housekeeping, genes are continuously expressed as they encode proteins vital for fundamental cellular processes. These include enzymes for glycolysis, ribosomal components for protein synthesis, and proteins involved in DNA replication. Their constant...
Master Transcription Regulators02:23

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...

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A Workflow for the Quantitative Assessment of the Endophytic and Epiphytic Bacterial Microbiomes of the Bark of Populus trichocarpa
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UV-B-responsive microRNAs in Populus tremula.

Xiaoyun Jia1, Ligang Ren, Qi-Jun Chen

  • 1Center for Agricultural Biotechnology, Shanxi Agricultural University, Taigu 030801, China.

Journal of Plant Physiology
|July 25, 2009
PubMed
Summary

This study identifies 24 microRNAs (miRNAs) that respond to UV-B stress in Populus tremula plants. These findings reveal novel regulatory networks involved in plant stress responses.

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RNA Blot Analysis for the Detection and Quantification of Plant MicroRNAs
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RNA Blot Analysis for the Detection and Quantification of Plant MicroRNAs

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Area of Science:

  • Plant Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • MicroRNAs (miRNAs) are key regulators of gene expression at the post-transcriptional level.
  • Understanding plant responses to environmental stresses like UV-B is crucial for agriculture and ecology.

Purpose of the Study:

  • To identify UV-B stress-responsive miRNAs in Populus tremula.
  • To elucidate the regulatory roles and networks of these miRNAs in plant stress response.

Main Methods:

  • Expression profiling using an in-house miRNA filter array.
  • Validation of miRNA and target gene expression via RNA blotting and qRT-PCR.
  • Promoter analysis to identify regulatory elements.

Main Results:

  • Identified 24 UV-B-responsive miRNAs (13 up-regulated, 11 down-regulated) in Populus tremula.
  • Verified six miRNAs and their target genes, which include transcription factors and phytohormone-related proteins.
  • Promoter analysis revealed light-relevant cis-elements, suggesting complex regulatory mechanisms.

Conclusions:

  • MicroRNAs are integral regulators of plant responses to UV-B stress.
  • A potential regulatory network mediated by UV-B-responsive miRNAs in Populus tremula was modeled.
  • These findings offer new insights into the ubiquitous role of miRNAs in plant stress adaptation.