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Related Experiment Video

Updated: Jun 21, 2026

Gene Trapping Using Gal4 in Zebrafish
13:34

Gene Trapping Using Gal4 in Zebrafish

Published on: September 29, 2013

Optimized Gal4 genetics for permanent gene expression mapping in zebrafish.

Martin Distel1, Mario F Wullimann, Reinhard W Köster

  • 1Helmholtz Zentrum München, Institute of Developmental Genetics, Ingolstädter Landstrasse 1, 85764 Neuherberg, Germany.

Proceedings of the National Academy of Sciences of the United States of America
|July 25, 2009
PubMed
Summary

Researchers optimized the Gal4-UAS system in zebrafish using an improved activator (KalTA4). This enables precise control over gene expression for studying gene function and developmental processes, including creating stable genetic lines.

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Area of Science:

  • Developmental Biology
  • Genetics
  • Neuroscience

Background:

  • The Gal4-UAS system is crucial for conditional gene expression in model organisms.
  • Adapting this system for zebrafish has opened new avenues for genetic research.
  • Optimizing existing tools is essential for advancing zebrafish functional genomics.

Purpose of the Study:

  • To systematically optimize the Gal4-UAS genetic system in zebrafish.
  • To develop novel tools for spatiotemporal control of gene expression.
  • To enable advanced genetic studies, including fate mapping and dosage analysis.

Main Methods:

  • Establishment and characterization of an optimized Gal4-activator, KalTA4.
  • Quantitative analysis of KalTA4-mediated transgene activation based on UAS copy number.

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Last Updated: Jun 21, 2026

Gene Trapping Using Gal4 in Zebrafish
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Published on: September 29, 2013

Live Imaging of Innate Immune and Preneoplastic Cell Interactions Using an Inducible Gal4/UAS Expression System in Larval Zebrafish Skin
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Live Imaging of Innate Immune and Preneoplastic Cell Interactions Using an Inducible Gal4/UAS Expression System in Larval Zebrafish Skin

Published on: February 3, 2015

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  • Tol2 transposon-mediated enhancer trap screen for central nervous system expression.
  • Development and characterization of a self-maintaining effector strain, Kaloop.

    • Main Results:

    • KalTA4 provides reliable and tunable transgene activation in zebrafish.
    • A collection of red fluorescent KalTA4 activator strains with CNS expression was generated.
    • The Kaloop strain allows for sustained transgene expression from transient embryonic induction.
    • Kaloop was used to trace the developmental origin of the adult secondary octaval nucleus.

    Conclusions:

    • The optimized KalTA4 system significantly enhances Gal4-UAS genetics in zebrafish.
    • Kaloop enables permanent spatiotemporal genetic fate mapping and targeted gene expression.
    • This work provides powerful new tools for dissecting complex biological processes in zebrafish.