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A simple, sensitive assay for the spermicide nonoxynol-9 in biological fluids by high-performance liquid
G J Beck1, D Kossak, S J Saxena
1VLI Corporation, Irvine, CA 92714.
Journal of Pharmaceutical Sciences
|November 1, 1990
Abstract:
A new high-performance liquid chromatographic technique has been developed to quantitate nonoxynol-9 in serum, urine, and vaginal fluid. The method is rapid, involves minimal sample preparation, and can be used to analyze a large number of biological fluid samples. The assay elutes a single nonoxynol-9 peak with no interfering components. This was accomplished using a 10-microns pelicular packed amine column, a normal-phase solvent system, and fluorescence detection. Nonoxynol-9 levels as low as 0.23 micrograms/mL in urine can be detected.
Keywords:
BiologyClinical ResearchContraceptionContraceptive Agents--analysisData AnalysisExaminations And DiagnosesFamily PlanningGenitaliaGenitalia, FemaleHemic SystemHuman VolunteersLaboratory Examinations And DiagnosesLaboratory ProceduresNonoxynol-9--analysisPhysiologyResearch MethodologySpermicidal Contraceptive Agents--analysisUrogenital SystemVagina