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Membrane Remodeling of Giant Vesicles in Response to Localized Calcium Ion Gradients
Published on: July 16, 2018
Temperature-dependent vesicle response to surface topography.
Susan D Gillmor1, Julia J Heetderks, Paul S Weiss
1Department of Chemistry, George Washington University, 725 21st Street, N.W., Washington, DC 20052, USA. sdgill@gwu.edu
The Journal of Physical Chemistry. B
|July 29, 2009
Summary
Giant unilamellar vesicles deform on surfaces and deflate when cooled with solutes. A transient defect pore model explains this vesicle deflation across tested temperatures.
Area of Science:
- Biophysics
- Materials Science
Background:
- Vesicle deformation on surfaces is influenced by temperature.
- The mechanism of vesicle deflation during cooling with low-permeability solutes remains unclear.
Purpose of the Study:
- To investigate giant unilamellar vesicle (GUV) deformation and deflation on surface topography.
- To quantify GUV cooling dynamics over a range of temperatures.
Main Methods:
- Utilized giant unilamellar vesicles (GUVs) on patterned surfaces.
- Quantified vesicle cooling and deformation across various temperatures.
- Analyzed volume constraints to determine deflation mechanisms.
Main Results:
- Vesicle deflation was observed over the studied temperature range.
- The cooling process was quantified in the presence of a low-permeability solute.
- Volume constraints supported a specific deflation model.
Conclusions:
- A transient defect pore model accurately describes vesicle deflation during cooling on topography.
- Temperature significantly impacts vesicle behavior and deflation mechanisms.
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