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Updated: Jun 21, 2026

Assessing Replication and Beta Cell Function in Adenovirally-transduced Isolated Rodent Islets
Published on: June 25, 2012
Production and characterization of the recombinant Islet Neogenesis Associated Protein (rINGAP)
Béatrice Assouline-Thomas1, Amélie Pilotte, Maria Petropavlovskaia
1Department of Surgery, McGill University, Montréal, Que., Canada H3G1A4. Beatrice.Assouline@mail.mcgill.ca
Abstract:
Islet Neogenesis Associated Protein (INGAP) is implicated in pancreatic islet neogenesis. INGAP peptide, a pentadecapeptide comprising amino acids 104-118, reverses diabetes in rodents and improves glucose homeostasis in patients with diabetes. The mechanism of INGAP action is unknown, but such studies would benefit from the availability of the full-length recombinant protein (rINGAP). Here we report the production of rINGAP from 293-SF cells following lentiviral transduction, and its characterization by MALDI-TOF and Q-TOF Mass Spectrometry, and HPLC. Importantly, we show that rINGAP exhibits 100x the bioactivity of INGAP peptide on a molar basis in an in vitro assay of human islet regeneration.
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