Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Scanning Electron Microscopy01:07

Scanning Electron Microscopy

A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
Fundamental Principles
Accelerated...
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Salvianolic acid B alleviates depression-like behaviors by reducing neuronal injury and promoting neurogenesis in a manner associated with JAK-STAT signaling pathway inhibition.

Frontiers in neurology·2026
Same author

[Polydatin attenuates high copper induced damage in Atp7b knockout neural stem cells by regulating PPARG mediated ferroptosis].

Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences·2026
Same author

ProBDNF promotes LPS-induced pro-inflammatory polarization of alveolar macrophages via the Notch1 signaling pathway.

Immunologic research·2026
Same author

Financial Feasibility of BPaL(M) Under Pretomanid Price Scenarios - China, 2023-2024.

China CDC weekly·2026
Same author

Curdlan-Reinforced Chitosan/Polyacrylate Interpenetrating Hydrogels with Enhanced Mechanical Stability for Gastric Retention and pH-Responsive Drug Release.

Gels (Basel, Switzerland)·2026
Same author

Discovery of a CI-994 derivative as a dual modulator of class I HDACs and Wnt/β- catenin signaling for Alzheimer's disease therapy.

bioRxiv : the preprint server for biology·2026
Same journal

Deep Learning Based Framework for Detection and Classification of Leukemia Using Microscopic Images.

Microscopy research and technique·2026
Same journal

In Situ TEM Observation of Phase Transformation Nucleation at the Near-Surface of Synthetic Aragonite.

Microscopy research and technique·2026
Same journal

Morpho-Anatomical and HPTLC Investigations of Lysimachia nummularia L. (Primulaceae) Grown in Switzerland.

Microscopy research and technique·2026
Same journal

Macroscopic, Histological and Ultrastructural Features of the Tongue of the Anatolian Wild Boar (Sus scrofa libycus).

Microscopy research and technique·2026
Same journal

Ultrastructural Insights Into the Reproductive Anatomy and Eggs of Cotton Pink Bollworm, Pectinophora gossypiella Saunders (Lepidoptera: Gelechiidae).

Microscopy research and technique·2026
Same journal

Automated FRET Analysis for Enhanced Characterization of Protein-Protein Interactions.

Microscopy research and technique·2026
See all related articles

Related Experiment Video

Updated: Jun 13, 2026

Implementation of a Nonlinear Microscope Based on Stimulated Raman Scattering
09:13

Implementation of a Nonlinear Microscope Based on Stimulated Raman Scattering

Published on: July 6, 2019

Externally Controlled In Situ SEM: Multi-Rate Scanning With Signal Regulation and Spatiotemporal Fusion.

Jieping Ding1, Ling'en Liu1, Ni Wang2

  • 1School of Materials Science and Engineering, Beijing University of Technology, Beijing, China.

Microscopy Research and Technique
|June 11, 2026
PubMed
Summary
This summary is machine-generated.

High-temperature in situ scanning electron microscopy (SEM) imaging is improved by a new system that reduces thermal electron noise. This rapid scanning approach enhances image quality and speeds up data acquisition for materials analysis.

Keywords:
image enhancementimaging systemin situ SEMmulti‐rate scanning

More Related Videos

In vivo Imaging of Biological Tissues with Combined Two-Photon Fluorescence and Stimulated Raman Scattering Microscopy
09:06

In vivo Imaging of Biological Tissues with Combined Two-Photon Fluorescence and Stimulated Raman Scattering Microscopy

Published on: December 20, 2021

Related Experiment Videos

Last Updated: Jun 13, 2026

Implementation of a Nonlinear Microscope Based on Stimulated Raman Scattering
09:13

Implementation of a Nonlinear Microscope Based on Stimulated Raman Scattering

Published on: July 6, 2019

In vivo Imaging of Biological Tissues with Combined Two-Photon Fluorescence and Stimulated Raman Scattering Microscopy
09:06

In vivo Imaging of Biological Tissues with Combined Two-Photon Fluorescence and Stimulated Raman Scattering Microscopy

Published on: December 20, 2021

Area of Science:

  • Materials Science
  • Microscopy
  • Engineering

Background:

  • In situ scanning electron microscopy (SEM) is vital for studying material properties at high temperatures.
  • High-temperature environments introduce thermal electrons and noise, distorting SEM images and hindering analysis of phase transitions and deformations.

Purpose of the Study:

  • To develop an advanced in situ image acquisition system to overcome image distortion caused by thermal electrons and noise in high-temperature SEM.
  • To improve the quality and speed of high-temperature SEM imaging for dynamic microstructure characterization.

Main Methods:

  • Implemented an externally controlled in situ image acquisition system featuring multi-rate scanning to minimize thermal electron interference.
  • Incorporated a dual-stage signal regulator to counteract signal attenuation during high-speed scanning.
  • Integrated an image fusion algorithm for effective noise suppression while preserving image details.

Main Results:

  • The system successfully acquired high-quality SEM images at 1050°C with an acquisition time of 1.5 seconds, significantly faster than traditional systems (3.5 seconds).
  • Synthesized images showed improved Structural Similarity Index (SSIM) and reduced Noise Quality Index (NIQE) between 900°C and 1050°C compared to conventional speeds.
  • Demonstrated reliable technical support for dynamic microstructure characterization of high-temperature materials.

Conclusions:

  • The developed hardware-algorithm solution effectively mitigates thermal electron noise and signal attenuation in high-temperature SEM.
  • This advancement enables high-quality, rapid in situ imaging, promoting SEM applications in extreme environments for materials research.