Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

iChip

iChip

The cultivation of environmental microorganisms has long been hindered by the inability to replicate complex native conditions in vitro. The isolation chip (iChip) addresses this limitation by facilitating the growth of previously uncultivable microorganisms through in situ incubation. Designed for high-throughput microbial cultivation, the iChip comprises hundreds of microchambers, each capable of housing a single microbial cell. These microchambers are loaded with a mixture of molten agar and...

Clamper Circuit

Clamper Circuit

A clamper circuit, also known as a DC restorer, represents a specialized variant of the rectifier circuit, notable for its method of taking the output across the diode rather than the capacitor. This configuration lends to several distinctive applications, particularly in handling square wave inputs.
Within this circuit, the diode's orientation prompts the capacitor to charge up to the level of the most negative peak of the input signal. Upon reaching this state, the diode ceases to conduct,...

Clipper Circuit

Clipper Circuit

A clipper circuit is a fundamental wave-shaping device that harnesses the unique properties of diodes to alter and control waveform characteristics. This technology is widely used in electronic devices, especially in television and radar communication systems, where it enhances waveform modulation in both transmitters and receivers.
The operation of a clipper circuit can be exemplified by analyzing a dual-clipper configuration setup that integrates two ideal diodes, each paired with a biasing...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Digital microfluidics with distance-based detection - a new approach for nucleic acid diagnostics.

Lab on a chip·2023

Same author

Rapid, sensitive, and low-cost detection of Escherichia coli bacteria in contaminated water samples using a phage-based assay.

Scientific reports·2022

Same author

A microfluidic device and instrument prototypes for the detection of <i>Escherichia coli</i> in water samples using a phage-based bioluminescence assay.

Lab on a chip·2022

Same author

Automated liquid handling robot for rapid lateral flow assay development.

Analytical and bioanalytical chemistry·2022

Same author

A Novel Malaria Lateral Flow Assay for Detecting Plasmodium falciparum Lactate Dehydrogenase in Busia, Uganda.

The American journal of tropical medicine and hygiene·2022

Same author

A SARS-CoV-2 coronavirus nucleocapsid protein antigen-detecting lateral flow assay.

PloS one·2021

Same journal

Microfluidic rare cell analysis beyond counting: workflow design from enrichment to multi-omics.

Lab on a chip·2026

Same journal

A sperm racetrack to separate sperm by swim speed.

Lab on a chip·2026

Same journal

Controlled encapsulation and droplet size prediction in two-step microfluidic double emulsions.

Lab on a chip·2026

Same journal

A particulate blood-mimicking fluid with physiological biconcave geometry for microscale hemorheology.

Lab on a chip·2026

Same journal

Multicellular sensor arrays fabricated by capillary stamping for pattern-based odor discrimination.

Lab on a chip·2026

Same journal

A real-time microfluidic surveillance system for multiplex detection of heavy metal contamination in wastewater.

Lab on a chip·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 21, 2026

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

SlipChip.

Wenbin Du¹, Liang Li, Kevin P Nichols

¹Department of Chemistry and Institute for Biophysical Dynamics, The University of Chicago, 929 East 57th Street, Chicago, Illinois 60637, USA.

|July 29, 2009

Summary

This summary is machine-generated.

The SlipChip is a novel microfluidic device enabling multiplexed reactions without pumps or valves. This simple, low-cost technology facilitates exposing a single sample to numerous reagents in nanoliter volumes, ideal for resource-poor settings.

More Related Videos

Microfluidic Chip Fabrication and Method to Detect Influenza

Microfluidic Chip Fabrication and Method to Detect Influenza

Published on: March 26, 2013

Detection of a CDH1 Rare Transcript Variant in Fresh-frozen Gastric Cancer Tissues by Chip-based Digital PCR

Detection of a CDH1 Rare Transcript Variant in Fresh-frozen Gastric Cancer Tissues by Chip-based Digital PCR

Published on: February 5, 2018

Related Experiment Videos

Last Updated: Jun 21, 2026

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

Microfluidic Chip Fabrication and Method to Detect Influenza

Microfluidic Chip Fabrication and Method to Detect Influenza

Published on: March 26, 2013

Detection of a CDH1 Rare Transcript Variant in Fresh-frozen Gastric Cancer Tissues by Chip-based Digital PCR

Detection of a CDH1 Rare Transcript Variant in Fresh-frozen Gastric Cancer Tissues by Chip-based Digital PCR

Published on: February 5, 2018

Area of Science:

Microfluidics
Biotechnology
Chemical Engineering

Background:

Microfluidic devices often require complex components like pumps and valves.
Multiplexed reactions in small volumes are crucial for high-throughput screening and diagnostics.
Existing platforms can be expensive and difficult to operate, limiting accessibility.

Purpose of the Study:

To introduce and validate the SlipChip, a novel microfluidic device for multiplexed reactions.
To demonstrate the device's capability for performing reactions without pumps or valves.
To assess the feasibility of using the SlipChip in resource-limited environments.

Main Methods:

Fabrication of a two-plate microfluidic device (SlipChip) using glass.
Preloading nanoliter-scale wells in the bottom plate with reagents.
Utilizing a "slipping" motion between plates to initiate diffusion and reaction.
Testing stability, loading control, and cross-contamination with fluorescent dyes.
Demonstrating functionality through model membrane protein crystallization.

Main Results:

The SlipChip successfully performed multiplexed reactions on a nanoliter scale.
Stability of preloaded reagents and controlled loading were confirmed.
No cross-contamination was observed between reaction sites.
The device demonstrated functionality in a complex application (protein crystallization).
Fabrication is simple, requiring no bonding, and the device operates without pumps or valves.

Conclusions:

The SlipChip offers a simple, pump-and-valve-free approach for multiplexed microfluidic reactions.
Its ease of use and low-cost fabrication make it suitable for resource-poor settings.
The device is a valuable tool for applications requiring exposure of a sample to multiple reagents in small volumes, akin to a preloaded multi-well plate or liquid-phase microarray.