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Updated: Jun 21, 2026

High-Throughput Analysis of Non-Photochemical Quenching in Crops Using Pulse Amplitude Modulated Chlorophyll Fluorometry
Published on: July 6, 2022
Kinetic models of photosystem II should accommodate the effect of donor side quenching on variable chlorophyll A
Abstract:
Quantitative data on laser flash-induced variable fluorescence in the 100 ns to 1 ms time range (Belyaeva et al. in Photosynth Res 98:105-119, 2008) confirming those of others (Steffen et al. in Biochemistry 40:173-180, 2001, Biochemistry 44:3123-3132, 2005; Belyaeva et al. in Biophysics 51(6):976-990, 2006), need a substantial correction with respect to magnitude of the normalized variable fluorescence associated with single turnover-induced charge separation in RCs of PS II. Their data are conclusive with the involvement of donor side quenching, the release of which occurs with a rate constant in the range of tens of ms(-1), and presumed to be associated with reduction of Y(+)(z) by the OEC.
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