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Photonic immobilization of high-density protein arrays using Fourier optics

Esben Skovsen1, Ane B Kold, Maria Teresa Neves-Petersen

  • 1Department of Physics and Nanotechnology, Aalborg University, Denmark.

Proteomics
|July 29, 2009
PubMed

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Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...