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[Construction of Deinococcal bacteria-Escherichia coli shuttle vector and expression of Luciferase gene]
Zhenli Tu1, Rujie Zhong, Jiagang Wang
1College of Animal Sciences, Zhejiang University, Hangzhou 310029, China. tuzl@zju.edu.cn
Objective:
To express Luciferase gene in Escherichia coli through developed Deinococcal bacteria-E. coli shuttle expression vector.
Methods:
The D. bacteria-E. coli shuttle expression vector pZT17 was constructed based on plasmids of pUE30, pGBM5 and pKatCAT. Then pZT17 with lux + from Photinus pyralis was used to transform into D. grandis and E. coli. The recombinant strains were induced separately.
Results:
Based on a small cryptic plasmid from Deinococcus radiopugnans, a shuttle vector between Escherichia coli and Deinococcal bacteria was constructed. The plasmid vector could stably aintained in Deinococcus grandis under non-selective conditions. Moreover, it is showed that a luciferase gene was highly expressed both observed in D. grandis and E. coli.
Conclusions:
The D. bacteria-E. coli shuttle vector was constructed successfully, the developed shuttle vector makes it possible to induce expression of DNA damage and repair gene from Deinococcus species.
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