Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
Next-generation Sequencing03:00

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.
DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Vertebrate Genomes Project Phase I: A global reference genome resource.

bioRxiv : the preprint server for biology·2026
Same author

Author Correction: Regulatory T cells in the mouse hypothalamus control immune activation and ameliorate metabolic impairments in high-calorie environments.

Nature communications·2026
Same author

HM-DyadCap - capture and mapping of 5-hydroxymethylcytosine/5-methylcytosine CpG dyads in mammalian DNA.

Nucleic acids research·2026
Same author

Unraveling Synthetase's Mode of Action: The Pyrrolysyl-tRNA Synthetase Dimer Uses Secondary Binding Sites in the Cell.

Angewandte Chemie (International ed. in English)·2026
Same author

Intracellular expression of a fluorogenic DNA aptamer using retron Eco2.

eLife·2026
Same author

Assessing the readiness of Oxford Nanopore sequencing for clinical genomics applications.

Genome research·2026

Related Experiment Video

Updated: Jun 21, 2026

Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies
13:24

Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies

Published on: April 11, 2016

Microarray-based multicycle-enrichment of genomic subsets for targeted next-generation sequencing.

Daniel Summerer1, Haiguo Wu, Bettina Haase

  • 1febit biomed gmbh, 69120 Heidelberg, Germany. daniel.summerer@febit.de

Genome Research
|July 30, 2009
PubMed
Summary

This study introduces an iterative hybridization method using microfluidic biochips to significantly improve DNA sequence enrichment for next-generation sequencing. This approach enhances coverage depth and breadth for targeted genomic regions, enabling more reliable genetic analysis.

More Related Videos

High-throughput Identification of Gene Regulatory Sequences Using Next-generation Sequencing of Circular Chromosome Conformation Capture (4C-seq)
09:06

High-throughput Identification of Gene Regulatory Sequences Using Next-generation Sequencing of Circular Chromosome Conformation Capture (4C-seq)

Published on: October 5, 2018

Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq
06:24

Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq

Published on: March 12, 2021

Related Experiment Videos

Last Updated: Jun 21, 2026

Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies
13:24

Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies

Published on: April 11, 2016

High-throughput Identification of Gene Regulatory Sequences Using Next-generation Sequencing of Circular Chromosome Conformation Capture (4C-seq)
09:06

High-throughput Identification of Gene Regulatory Sequences Using Next-generation Sequencing of Circular Chromosome Conformation Capture (4C-seq)

Published on: October 5, 2018

Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq
06:24

Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq

Published on: March 12, 2021

Area of Science:

  • Genomics
  • Molecular Biology
  • Biotechnology

Background:

  • Next-generation sequencing (NGS) requires efficient methods for enriching specific genomic DNA sequences.
  • Current enrichment techniques often lack sufficient coverage depth for reliable base calling across target regions.

Purpose of the Study:

  • To develop and validate a novel strategy for multiplying sequence enrichment performance.
  • To improve the depth and breadth of coverage for targeted genomic regions using iterative hybridization.

Main Methods:

  • Utilized two iterative cycles of hybridization with microfluidic Geniom biochips for DNA sequence enrichment.
  • Applied Illumina sequencing technology to enriched cancer-related genes (BRCA1, TP53) and dbSNP regions.
  • Analyzed enrichment factors, coverage depth, uniformity, and SNP calling accuracy.

Main Results:

  • Achieved high enrichment factors (up to 1062-fold) and average coverage depths (470-fold).
  • Demonstrated high coverage uniformity, with >86% of target regions covered at 20-fold or higher.
  • Exhibited excellent concordance in SNP calling accuracy compared to the reference sequence.

Conclusions:

  • The iterative hybridization strategy significantly enhances DNA sequence enrichment for NGS.
  • This method provides deep and uniform coverage, enabling reliable analysis of targeted genomic regions.
  • The approach is effective for sequencing cancer-related genes and dbSNP regions, with potential applications in various genomic studies.