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The Tumor Microenvironment

The Tumor Microenvironment

Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...

The Tumor Microenvironment

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Related Experiment Video

Updated: Jun 21, 2026

Isolation and Culture Expansion of Tumor-specific Endothelial Cells

Isolation and Culture Expansion of Tumor-specific Endothelial Cells

Published on: October 14, 2015

Tumor endothelial cells join the resistance.

Andrew C Dudley¹, Michael Klagsbrun

¹Vascular Biology Program, Departments of Surgery and Pathology, Harvard Medical School, Boston, MA 02115, USA. andrew.dudley@childrens.harvard.edu

Clinical Cancer Research : an Official Journal of the American Association for Cancer Research

|July 30, 2009

Summary

Tumor blood vessels are surprisingly complex. Tumor-specific endothelial cells show reduced sensitivity to anti-cancer drugs compared to normal cells, impacting antiangiogenesis therapy effectiveness.

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Area of Science:

Oncology
Vascular Biology
Cancer Therapeutics

Background:

Antiangiogenesis research aims to inhibit tumor blood vessel formation.
Tumor vasculature is unexpectedly complex and dynamic.
Previous assumptions about tumor blood vessels require revision.

Purpose of the Study:

To investigate the sensitivity of tumor-specific endothelial cells to cytotoxic and antiangiogenic drugs.
To understand the implications of endothelial cell heterogeneity in cancer treatment.

Main Methods:

Comparative drug sensitivity assays.
Analysis of endothelial cell responses to various therapeutic agents.
Utilizing models to study tumor-specific endothelial cell behavior.

Main Results:

Tumor-specific endothelial cells exhibit significantly lower sensitivity to cytotoxic drugs.
These specialized cells are also less responsive to antiangiogenic agents.
This differential sensitivity challenges existing therapeutic strategies.

Conclusions:

Endothelial cell differences between tumors and normal tissues are critical.
The reduced drug sensitivity of tumor endothelial cells may limit the efficacy of antiangiogenesis therapies.
Further research is needed to develop strategies overcoming this resistance.