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An unusual phosphodiesterase activity towards p-nitrophenylphosphorylcholine present in rat brain membranes
1Department of Biochemistry, University of Manitoba, Winnipeg, Canada.
Neurochemical Research
|October 1, 1990
Abstract:
A phosphodiesterase activity present in rat brain membranes has been examined utilizing p-nitrophenylphosphorylcholine as the substrate. This enzyme activity has a pH optimum of 8.5, is stimulated by a variety of free fatty acids, requires either Zn+2 or Ca+2 and is relatively stable to heating at 75 degrees C for 7.5 minutes. These properties appear to distinguish this particular activity from those previously reported for alkaline phosphatase, nonspecific phosphodiesterase, phosphodiesterases I and II, lecithinase, and sphingomyelinase.